Fig. 4

Sperm proteins are deregulated in XY^RIIIqdel mice. A Sperm lysates from the wild type XY^RIII strain and the mutant XY^RIIIqdel mice were separated by 2D-PAGE in the pI ranges of 4–7 and 5–8 on 8–20% gradient gels (see also Additional file 10: Fig. S5A). Five differentially expressed proteins, D1 to D5 - Calreticulin, SPINK2 variant 2, SPINK2 variant 3, SOD and FABP9 respectively, were identified by mass spectrometry analysis in the 4–7 pI range, of which 4 were upregulated (upward arrow) and one downregulated (downward arrow). Three proteins, A, B and C—spot A, SDF2L1 and MAST were not detectable in the 5–8 pI range in XY^RIIIqdel compared to the XY^RIII sperm lysate. B List of the differentially expressed proteins identified in the proteomics screen is given in the table along with MS tags and N-terminal sequences. Genes corresponding to all the differentially expressed proteins in XY^RIIIqdel localized to different autosomes