Fig. 7

Unique features of grass FT proteins. Diagram showing changes in otherwise conserved residues in FT protein clades from the Poaceae. A total of 33 residues are shown along the top, these are either residues where Angisoperm FT proteins (without the monocots)(AnFT) have a unique amino acid relative to other EuPEBP proteins, or otherwise conserved amino acids in important motifs (shaded pink), 14-3-3a and b (60-64 and 95-101), P-loop (128-140) and LYN triad (150-152). The consensus sequence within non-angiosperm MFT (NaMFT), angiosperm MFT (AnMFT), angiosperm TCB (AnTCB) and AnFT at each residue is shown in rows two to five. Amino acids characteristic for NaMFT are shaded yellow, those for AnMFT are shaded orange, those for TCB are shaded green, and those for AnFT are shaded purple. Residues shared across all MFT proteins are shaded beige. The darker the shade, the higher the degree of conservation within the clade. Residues that are not characteristic are shaded grey; if there is no consensus, a backslash (\) is shown. The following 15 rows indicate the consensus amino acids present at the same residues in the different FT clades in the Poaceae. Blue shading indicates that the consensus matches the consensus for EuFT. Dark blue = highly conserved in clade (> 90%), mid-blue = conserved (> 75%), light blue = consensus (> 50%). Red shading indicates that the consensus does not match the consensus for EuFT. Dark red = highly conserved in clade (> 90%), mid red = conserved (> 75%), pink = consensus (> 50%), or where backslash is shown, no consensus (but therefore still distinct from EuFT), and where # is shown, residue is completely absent