Fig. 5

FIB/SEM analysis reveals connecting microtubular fiber between central mitosomes and the axonemes of caudal flagella. A The frontal plane image of the central mitosomes of interphase Giardia between the longitudinally sectioned axonemes as reconstructed from the transversal FIB/SEM images, AXO—axoneme, red arrowhead—microtubular fiber, N—nuclei. B The illustrative transversal section shows two fibers (red arrowheads) connecting central mitosomes and axonemes of caudal flagella. M—mitosomes. C The reconstruction of the FIB/SEM analysis depicting the overall architecture of the microtubular fiber (yellow) between central mitosomes (red) and axonemes of caudal flagella (dark blue). Axonemes of anterolateral flagella are shown in light blue, N-nuclei. D The dimensions of the connecting fiber (n = 15 and n = 3 for the length and of the connector and the binding region, respectively). E Transmission electron micrographs show the detailed structure of the connecting microtubular fibers, which each consists of 3 to 4 microtubules. M-mitosomes