Fig. 3

Signaling programs in HSPC subsets. A Representative contour plots for pSTAT3, pAkt, and β-catenin in different HSPC subsets. Combined charts of dot plots, box plots, and fitting curves showing the frequency changes of the indicated protein positive cells in different LK cell subsets in mice. Fitting curves in different colors indicate the changes of different differentiation routes. B Combined charts of dot plots, box plots, and fitting curves showing the frequency changes of p-p38, pSHP2, pRb, pEGFR, IκBα, and pSTAT1 positive cells in different LK cell subsets in mice. C Schematic diagram showing the signaling protein changes during HSC differentiation. D Heatmaps showing the normalized mean expression of the indicated signaling proteins in pRb⁻ and pRb⁺ HSPC subsets from mice. p values were calculated with paired Student’s t test. n = 4 mice in each group. *p < 0.05, **p < 0.01