Fig. 5

Characterization and changes of LSK cell clusters during hematopoietic recovery. A SPADE tree describing 50 minor LSK cell clusters of one representative mouse colored by the median expression of the indicated markers. LSK subsets are gated with a grey color. B Heatmaps showing the normalized median expression of the indicated markers in 50 minor LSK cell clusters in mice after different days of 5-FU treatment (n = 4 mice in each group). C Heatmaps displaying the differences in proteins’ expression levels in between LSK cells clusters in mice after the indicated days of 5-FU treatment. D Bar plots showing the frequency changes of LSK cell clusters (nodes) in mice after different days of 5-FU treatment. n = 4 mice in each group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. p values were calculated with Student’s t test