Fig. 1

Large-scale analysis. a Take a 384-format plate with quantified yeast colony sizes for example, this plate could be a result from the screening of a 383 TF-prey batch (from green, yellow, red, to purple) with a negative control/reference (blue) against a DNA-bait. The numbers on the plate represent the size values of each colony for the TF-prey batch against the DNA-bait. The value of each TF-DNA combination would be compared to the reference value. If the value of a TF-DNA combination is higher than the reference value, the TF-prey would be regarded as a positive, such as the 1st TF. On the opposite, when the value of a TF-DNA combination is lower than the reference value, the TF-prey would be interpreted as a negative, such as the 383th TF. The TF-DNA combinations can be increased into b multiple TF-prey batches against multiple DNA-baits (from i to iii). c Multiple TF-prey batches against multiple DNA-baits can be integrated with different parameters, such as different culturing days to generate dramatically increased numbers of colony size values