Fig. 1

The mouse model. A The experimental procedure and HE staining of subcutaneously transplanted tumors. One single cell was 3D-cultured in a 96-well plate to grow organoids (see “Methods” for details). Single cell-derived organoids were taken to separate single cells, and RNA and DNA were separately extracted from the different single cells of multiple organoids and then sequenced. The numbers of cells for RNA and DNA sequencing in boxes are those obtained after quality control of data. The culture experiment from intestinal crypts to T3 organoids was performed once. B Variant allele frequencies of mutations found in the significantly mutated genes of colorectal cancer by bulk-cell DNA sequencing. See Additional file 1: Figure S1 for the annotations of the mutations