Fig. 4
From: Self-organising human gonads generated by a Matrigel-based gradient system
Expression of steroidogenic pathway enzymes in the 3-LGS. A Immunolabelling of steroidogenic enzyme markers StAR (red) and CYP17A1 (green) revealed interstitial expression in all testicular organoids (TO) (representative organoid image from 8 wpc embryonic tissue sample). In vivo control male (M) 8 wpc testis. B No nuclear androgen receptor (AR) (green) expression in the interstitial compartment (representative image from 8 wpc TO sample) was observed. No 17-HSDβ3 (red) expression was detected. In vivo control male (M) 8 wpc and adult testis. Negative IgG isotype controls (rabbit (rb) (red) and mouse (ms) (green)). All organoid images from day 14 culture samples. Scale bars, 50 μm (insets, 10 μm)