Fig. 3
From: PACmn for improved optogenetic control of intracellular cAMP
Behavioral assessment of bPAC variants in Drosophila. A Mechanical nociceptors (C4da sensory neurons) expressing bPACs (grey, ppk-GAL4 driver). The soluble R278A and F198Y variants and Glyco-Venus-bPAC(S27A) are located in the cell body and dendrites. CD8-Venus-bPAC(wt) is confined to the somatic region of C4da neurons. B Nocifensive behavior in groups of larvae expressing bPAC variants in C4da neurons in the dark (black bar) or blue light (blue bar). Note that control larvae exhibited no nocifensive behavior except in response to von Frey filament stimulation and larvae expressing unmodified bPAC showed nocifensive behavior in the dark