Fig. 4

Nup54 promoter region is diverged in closely related species. A Sequence alignment of the Nup54 promoter region from closely related species. Nucleic acids deviating from D. melanogaster are indicated in black. Transcribed parts of the DUBAI 3′UTR and the Nup54 5′UTR, as well as the Nup54^QB62 allele are underlined. B Plot of cumulative differences along the sequence (G) between the relative occurrences of nucleotide changes and their position from the alignment of the gene region around Nup54 between D. melanogaster and D. simulans. Positions in the alignment with significant stretches of substitutions (hotspots) are identified by solid lines. The deletion in the Nup54^QB62 allele is indicated by a circle with a dashed line. The data underlying the presented graphs are in Additional file 6