Fig. 6

Conservation of Kr-h1 phosphorylation in other insects. A Western blot showing Kr-h1 phosphorylation in penultimate instar larvae of Bombyx mori, Tribolium castaneum, and Drosophila melanogaster, respectively. B Luciferase reporter assays after co-transfection of pGL4.10-4×BmE93^{-2844 to -2827} with pAc5.1/Flag-BmKr-h1, pAc5.1/Flag-BmKr-h1^S76A, pAc5.1/Flag-BmKr-h1^S76D or pAc5.1/Flag vector control into S2 cells. Methoprene was applied at 10 μM. C Luciferase reporter assays using S2 cells co-transfected with pGL4.10-4×TcE93 ^{-50 to -33} with pAc5.1/Flag-TcKr-h1, pAc5.1/Flag-TcKr-h1^S124A, pAc5.1/Flag-TcKr-h1^S124D, or pAc5.1/Flag. D Luciferase reporter assays using S2 cells co-transfected with pGL4.10-4×DmE93^{-2095 to -2078} with pAc5.1/Flag-DmKr-h1, pAc5.1/Flag-DmKr-h1^S255A, pAc5.1/Flag-DmKr-h1^S255D or pAc5.1/Flag. Means labeled with different letters indicate significant difference at P<0.05. n=3