Fig. 1

Catsup is the Drosophila ortholog of hZIP7 and functions as a zinc transporter. A The eclosion defect from ubiquitous Catsup RNA interference (Da-Gal4>Catsup RNAi) could be effectively rescued by hZIP7 expression, but not by dZIP13 OE. Genotypes of the flies used were Da-Gal4> w¹¹¹⁸ (control); Da-Gal4>Catsup RNAi; Da-Gal4>Catsup RNAi, hZIP7; Da-Gal4>Catsup RNAi, dZIP13 OE. All values are presented as mean ± SEM; n = 6. ***p < 0.001. B The shortened lifespan of Catsup RNAi in the central nervous system was rescued by hZIP7, but not dZIP13 OE. Genotypes of the flies used were Elav-Gal4> w¹¹¹⁸ (control); Elav-Gal4>Catsup RNAi; Elav-Gal4>Catsup RNAi, hZIP7; Elav-Gal4>Catsup RNAi, dZIP13 OE. n = 6.C hZIP7, but not dZIP13 OE, restored the eye blackspots of Catsup RNAi flies. Genotypes of the flies used were Elav-Gal4> w¹¹¹⁸ (control); Elav-Gal4>Catsup RNAi; Elav-Gal4>Catsup RNAi, hZIP7; Elav-Gal4>Catsup RNAi, dZIP13 OE. n ≥ 6. D The ALP activity, indicated by absorbance at 405 nm, was increased in the heads of Catsup RNAi flies when driven by the pan-neuronal driver Elav-Gal4, suggesting that Catsup RNAi in neurons led to zinc elevation in the Golgi. Genotypes of the flies used were Elav-Gal4> w¹¹¹⁸ (control) and Elav-Gal4>Catsup RNAi. E, F The localization of Catsup in Chinese hamster ovary (CHOK1) cells. Catsup-FLAG was detected by anti-FLAG antibody. This immunofluorescence staining showed that Catsup partially co-localizes with the ER/Golgi in CHOK1 cells