Fig. 6
From: Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells
Med1 and Med15 regions induce the formation of optodroplets upon illumination by blue light. a–d Representative images during optodroplet activation in NIH3T3 cells expressing the following constructs: mCherrry-Cry2 (a), Med1(948-1157)-mCherry-Cry2 (b), NLS*-Med15(71-617)-mCherry-Cry2 (c), and Med15(618-789)-mCherry-Cry2 (d). t = 0 s indicates the starting point of blue light illumination. Time intervals between illuminating blue light and image acquisition are noted on each image. All scale bars are 5 μm. Similar results were obtained from three independent experiments. e Percentage of cells forming optodroplets after 30 s blue light stimulation at the same intensity. Numbers of cells observed were the following: mCherry-Cry2: 19; Med1(948-1157): 42; Med15(1-617): 18; Med15 (618-789): 26. * and *** indicates p < 0.05 and p < 0.001 in Fisher’s exact test, respectively