Fig. 1

Twenty-four-hour periodicity in the transcriptomes of adult Schistosoma mansoni. A Schematic showing the collection of pooled worm samples from three mice every 4 h over 44 h, used to generate three RNA-seq time-series datasets: one for whole female worms, once for whole male worms and one for male heads. B In total, 209 diel genes were identified in S. mansoni (JTK_Cycle BH.Q < 0.01), with some showing 24-h periodicity in more than one dataset. C Expression heatmaps of diel genes. Each row represents a gene whose transcripts oscillate with ∼24 h periodicity, ordered vertically by phase. D Histograms of rhythmic daily fold changes (FC) in transcript abundance. E Histograms showing the distribution in peaks of expression in diel genes in both sexes and in male heads