Fig. 1
Neighborhood-Joining phylogenetic tree and expression analysis of wheat genes encoding VIH. A Neighborhood-Joining phylogenetic tree of PP-InsP5 proteins. The full-length amino acid sequences of VIH proteins from various taxonomic groups were used for the construction of phylogeny using MEGA7.0. The number represents the bootstraps values (1000 replicates). For construction of evolutionary history was inferred by Minimum Evolution method using 14 amino acid sequences spanning from all the wheat VIH homoeologs (TaVIH1 and TaVIH2), rice (Os01t04777700; Os03t0689100), Arabidopsis thaliana (NP_568308; NP_186780), human (HsVIP1-NP_001124330 and HsVIP2-NP_001263206), yeast (VIP1-NP_013514) and Dictyostelium discoideum (DDBXP638433). B TaVIH1 and TaVIH2 in different tissues of a wheat plant. The cDNA was prepared from 2 μg of DNA-free RNA isolated from root, stem, leaf, and flag leaf tissues of a 14 DAA plant as a template. C Quantitative expression analysis of TaVIH genes at different seed maturation stages (7, 14, 21, and 28 days after anthesis and; D Expression in the tissue of 14 DAA seed (aleurone, Al; endosperm, En; embryo, Em; glumes, Gl and rachis, Ra. For qRT-PCR, cDNA was prepared from 2 μg of DNA-free RNA isolated from respective tissues. TaARF was used as an internal control for the normalization of Ct values. Standard deviation was calculated along with its level of significance at p < 0.05 (*) with respect to the first tissue