Fig. 9

Heatmap expression analysis of gene families in Col-0(Ev) overexpressing TaVIH2-3B Arabidopsis (#Line4 and 6). A Heatmaps for expression patterns (as Z-scores) for genes DE in both transgenic lines w.r.t. Col-0(Ev), encoding for the genes involved in cell-wall homeostasis. B ABA biosynthesis-related pathway genes. C DREB encoding genes. D cytochrome P450 (CYPs) genes. Increasing intensities of brown and blue colors represent the comparatively low and high expression for each gene, as depicted by the color scale. Normalized expression counts were used to plotting the expression as Z-scores using heatmap. Two functions from the gplots package (Warnes et al., 2005) in R. Genes encoding for respective pathways were extracted using MapMan (Thimm et al., 2004). R1, R2, and R3 represent the biological replicates for the RNAseq analysis of the individual lines