Fig. 4.
LTP is not induced at CA3-CA1 synapses of PrnpZH3/ZH3 mice, and the HFS presentation generates epileptic seizures. a,b Evolution of fEPSP1 evoked in the CA1 region by paired-pulsed stimulation of Schaffer collaterals for the Prnp+/+ (a) and PrnpZH3/ZH3 (b) mice after the HFS session. Data are presented as the percentage of increase from baseline. Significant differences with baseline values are presented for fEPSP1 (#) and fEPSP2 (*) recordings in Prnp+/+ mice. c,d fEPSP mean slopes from Prnp+/+ (c) and PrnpZH3/ZH3 (d) mice before and after the HFS session. Data are presented as the percentage of increase from baseline values. e,f The insets show representative recordings (averaged 5 times) of fEPSPs evoked in Prnp+/+ (e) and PrnpZH3/ZH3 (f) mice by paired pulses (40 ms of inter-pulse interval) of similar intensities (2 × Threshold; ≈ 0.2 mA). g Representative examples of long (30 s) recordings carried out after an HFS stimulation protocol in Prnp+/+ and PrnpZH3/ZH3 Schaffer collaterals. Note the presence of a hippocampal seizure in the PrnpZH3/ZH3 mouse (arrows). h Percentage of mice that presented epileptic seizures following HFS presentations. i Seizure duration (s) following HFS. Data are presented as Mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, ##p < 0.01 and ###p < 0.001, two-way ANOVA + Bonferroni’s multiple comparisons test and Mann-Whitney U non-parametric test. Source data and individual data values are available in Additional file 10