Fig. 4

Repeat landscape (RL) and minimum spanning tree (MST) of two orthologous superfamilies of satellite DNA in O. decorus and L. migratoria (OSF02 and OSF12). a OSF02 showed the highest consensus turnover rate (CTR = 2.86) found among the 20 values estimated between orthologous pairs of families in both species. Note that OSF02 showed large amplification peaks in both species (green curve in O. decorus and red curve in L. migratoria) and that the MST showed complete separation of OdeSat02 and LmiSat03 sequences. b OSF12 showed the lowest CTR estimate (0.26 between OdeSat59 and LmiSat01) and the MST (on the right) reveals that the consensus DNA sequences of these two satDNA families showed only two differences. Also note in the RL (on the left) that the OdeSat59 curve is very close to zero, as this is the satDNA family in O. decorus showing the lowest abundance, indicating that OSF12 is represented in this species as relict remains which, by chance, almost coincide in consensus sequence with the most abundant subfamily in L. migratoria (LmiSat01A), thus evidencing extreme incomplete lineage sorting (see other cases in Additional file 2: Fig. S1)