Fig. 1
From: Highly reliable creation of floxed alleles by electroporating single-cell embryos
In vitro Cre assay predicts phasing of loxP insertions. a A target gene is floxed in introns 4 and 11 (arrowheads). b Schematic for in vitro Cre assay. The PCR amplicon by F2/R1 is unique to the circular product of Cre-mediated excision. c Two potential male founders, M32 and M45, are identified by NGS genotyping. d PCR products by F2/R1 resolved in an agarose gel. C: control, combined gDNA from animals positive for either 5′ or 3′ loxP site; NTC: no template control. e Genotyping of F1s from M32 and M45 confirms accuracy and sensitivity of the Cre assay