A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth

Table 2 Resistance to Cry1 toxin in larvae from the susceptible (DBM1Ac-S) and polygenic knockout strains

Toxins	Strains	N*	LC₅₀ (95% CL)^†	Slope ± SE	χ²(df)^‡	RR^§
Cry1Ac	DBM1Ac-S	210	0.73 (0.56–0.93)	2.02 ± 0.24	3.14(5)	1.0
	C2-3KO	210	3271.57 (2604.98–4198.52)	2.34 ± 0.28	4.80(5)	4482
	N1-3aKO	210	1040.23 (784.13–1463.05)	1.74 ± 0.23	1.58(5)	1425
	C-NKO	210	> 25,000^¶	—	—	> 34,247
Cry1Ab	DBM1Ac-S	210	0.70 (0.54–0.89)	2.18 ± 0.26	3.31(5)	1.0
	C2-3KO	210	413.77 (325.22–543.91)	2.19 ± 0.28	0.60(5)	591
	N1-3aKO	210	92.37 (71.44–120.50)	1.89 ± 0.22	1.20(5)	132
	C-NKO	210	> 3000^¶	—	—	> 4286
Cry1Aa	DBM1Ac-S	210	0.72 (0.48–1.03)	2.07 ± 0.24	6.16(5)	1.0
	C2-3KO	210	0.87 (0.66–1.12)	1.93 ± 0.22	3.16(5)	1.2
	N1-3aKO	210	0.93 (0.72–1.19)	2.04 ± 0.23	2.76(5)	1.3
	C-NKO	210	1.05 (0.81–1.36)	1.93 ± 0.22	3.46(5)	1.5

*Number of larvae tested (larvae of the control group not included)
^†Concentration of Cry1Ac toxin (mg/L) killing 50% of larvae and its 95% confidence limits (CL)
^‡The value of chi-square and degrees of freedom (df) were calculated by Polo Plus 2.0
^§RR: Resistance ratio (RR) calculated by LC₅₀ of the polygenic knockout strains divided by LC₅₀ of the susceptible DBM1Ac-S strain
^¶Both Cry1Ac and Cry1Ab toxins produced < 15% mortality in larvae from the C-NKO strain when examined with the Cry1Ac concentration of 2.5× 10⁴ mg/L and 3× 10³ mg/L

ISSN: 1741-7007