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Fig. 2 | BMC Biology

Fig. 2

From: A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

Fig. 2

Analysis of Rosa26 sgRNA off-target sites. No mutations were seen in the top 5 potential off-target sites. A List of top 10 potential off-target sites as determined by the Optimized CRISPR tool, base pair mismatches, and location in the genome. B–F Sequence chromatograms of each off-target site, showing the correct sequence for each of the n = 3 selected clones that were injected into blastocysts as compared to the wildtype sequence

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BMC Biology

ISSN: 1741-7007

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