Fig. 3

Germline Cre/FLP expression in RR5 adult mice to fluorescently label three cellular compartments in targeted cells. A–H RR5 mice bred to DBH^p2aFLP; Bactin-Cre mice express Cre ubiquitously and FLP in all noradrenergic (NA) neurons, thus marking only NA neurons, including the locus coeruleus (A–D) and A5 nucleus (E–H). I–P RR5 mice bred to Vgat_Cre; Vglut2-FLPo mice with dual recombinase expression imaged in the entopeduncular nucleus (I–L) and lateral habenula (M–P). In targeted cells that express both Cre and FLP, cell nuclei are labeled with TagBFP, cell soma and processes are labeled with sfGFP, and presynaptic contacts are labeled with tdTomato