Fig. 6

Retrograde viral FLP and germline Cre mediated expression of dual recombinases for tricistronic FP expression on RR5 background. A RR5; Vglut2^Cre mice were injected with retro-AAV-Ef1a-FLPo virus into the lateral hypothalamus. All Vglut2 neurons express Cre, but only those Vglut2 neurons projecting to the injected LHA will also express FLP. Marked double Cre/FLP-positive cells will express H2B-TagBFP highlighting the nucleus in blue, sfGFP filling the cell including the axon, and synaptophysin-tdTomato labeling presynaptic contacts. LHA: Lateral hypothalamic area. Vglut2 positive neurons that project to the LHA arise from the cingulate gyrus (B–E), piriform cortex (F–I), and medial habenula (J–M)