Fig. 5
From: DHA alleviates diet-induced skeletal muscle fiber remodeling via FTO/m6A/DDIT4/PGC1α signaling
DHA reduces m6A levels by increasing FTO protein expression. A LC-MS/MS quantification of the m6A/A in mRNA of GAS from mice fed HFD or HFD+DHA (n = 3). B LC-MS/MS quantification of the m6A/A in mRNA of myoblasts with different concentration of DHA (n = 3). Western blot analysis and quantification of major m6A methyltransferase and demethylase expression in C, D skeletal muscle and E, F myoblasts (n = 3). Different letters indicate significant differences between different doses of DHA treatment for 48 h (P < 0.05). Statistical analysis was performed using one-way ANOVA (Fig. 5B, F) and two-tailed paired Student’s t tests (Fig. 5A, D)