Fig. 6
From: DHA alleviates diet-induced skeletal muscle fiber remodeling via FTO/m6A/DDIT4/PGC1α signaling
DHA increases PGC1α expression through FTO. A Western blot analysis of major m6A methyltransferase and demethylase expression profile during myogenesis (n = 3). B Western blot analysis of FTO and PGC1α in fast-twitch muscle fiber (EDL) and slow-twitch muscle fiber (SOL) (n = 3). C The protein expression of PGC1α, D relative ATP content, and E mitogreen staining was tested in C2C12/siCtl and C2C12/siFto (n = 3). Scale bar: 200 μm. F Western blot analysis of FTO and PGC1α expression after DHA treatment and Fto silencing (n = 3). Statistical analysis was performed using two-tailed paired Student’s t tests