Fig. 8
From: DHA alleviates diet-induced skeletal muscle fiber remodeling via FTO/m6A/DDIT4/PGC1α signaling
YTHDF2 affects DDIT4 protein expression by regulating mRNA stability. A Western blot analysis of YTHDF2 and DDIT4 in control and Ythdf2 knockdown cells (n = 3). B qPCR analysis of Ythdf2 and Ddit4 in control and Ythdf2 knockdown cells (n = 3). C RIP-qPCR analysis of the interaction of Ddit4 with YTHDF2 (n = 3). D Lifetime of Ddit4 mRNA in control or YTHDF2 overexpression cells (n = 3). Relative mRNA levels were quantified by qPCR. Statistical analysis was performed using two-tailed paired Student’s t tests