Fig. 2

DIXDC1-KO mice had attenuated angiogenic potential. A Matrigel plug implanted into 8-week-old male WT and DIXDC1-KO mice for 7 days (murine VEGF-A 50 ng). n = 5 per group. B Quantification of hemoglobin(mg/ml) extracted from Matrigel plugs from WT and DIXDC1-KO mice. n = 5 per group. C CD31 immunostaining of Matrigel plugs implanted into 8-week-old WT and DIXDC1-KO mice for 7 days. Scale bars 50 μm. D Quantification of CD31 immunostaining of Matrigel plugs. E Murine aortic ring assay of 8-week-old WT and DIXDC1-KO mice (murine VEGF-A 50 ng). n = 5 per group. F, G, and H Quantification of number of sprouts, cumulative sprout length (in pixel), and sprouting area (percentage of control). All experiments were repeated on at least 3 different sets of WT and KO littermates. *p < 0.05, **p < 0.005, and ***p < 0.0001, by paired, 2-tailed Student’s t test and one-way ANOVA. Error bars represent the mean ± SD. Individual values can be found in Additional file 6: Fig 2