Fig. 2

Downregulation of epiblast-lineage markers in mouse blastocysts by JQ1 treatment (stages 3 to 4). A–N Immunohistochemical analysis of mouse blastocysts (stage 3) treated without JQ1 (control) (A–G) or with 5 μM JQ1 (H–N) for 6 h. NANOG (A, H), OTX2 (B, I), SOX2 (C, J), OCT3/4 (D, K), GATA6 (E, L), SOX17 (F, M), or CDX2 (G, N) (magenta), with DAPI (nuclei, blue) staining. O The ratio of numbers of NANOG-, OTX2-, SOX2-, OCT3/4-, GATA6-, SOX17-, or CDX2-expressing cells to DAPI-positive cells (nuclei) (two-tailed Mann-Whitney’s U-test; ***p < 0.001, *p < 0.05; n.s., not significant; OCT3/4, p = 0.476; GATA6, p = 0.914; SOX17, p = 0.954; CDX2, p = 0.476). Red lines indicate the mean values, and green lines represent the SE bars. Individual values of marker-expressing cells are provided in Additional file 15. The sample numbers analyzed for each experiment are indicated in Additional file 19. Scale bars, 40 μm in A–N