Fig. 3
Verticillium dahliae proteins VdSCP76 and VdSCP77 enhance host susceptibility by suppressing immunity. A, B ROS accumulation and callose deposition after transient co-expression of VdSCP76 or VdSCP77 with VdEG1 in Nicotiana benthamiana leaves from 4-week-old plants were determined by 3,3'-diaminobenzidine (DAB) and aniline blue staining, respectively. C Expression of resistance-related gene was detected using reverse transcription quantitative PCR at 2 days after co-agro-infiltration of VdSCP76 or VdSCP77 with VdEG1 in N. benthamiana leaves. In all above, single infiltrations of VdEG1 and GFP were used as the positive and negative controls, respectively. D Disease symptoms of Botrytis cinerea on N. benthamiana plants overexpressing VdSCP76 and VdSCP77. N. benthamiana leaves from a 5-week-old plants were inoculated with 10 μL of 1 × 107 conidia/ml B. cinerea, and symptoms analyzed at 4 days after inoculation. E,F Lesion development of B. cinerea on N. benthamiana leaves was evaluated from 4 days post-inoculation by determining the lesion diameter on leaves from six plants each. Fungal biomass of B. cinerea was determined by quantitative PCR. Error bars represent standard errors. *, **, and *** represent significant differences at P < 0.05, P < 0.01, and P < 0.001, respectively, between positive control VdEG1 and co-expression of VdEG1 with VdSCP76 or VdSCP77, according to one-way analysis of variance (ANOVA)