Fig. 1

Experimental setup. Individually housed rats exposed to LD12:12 were randomly assigned to one of the three groups: control (C), shift worker (W), or shift worker subjected to restricted feeding during work (WRF). All rats had ad libitum access to water. C rats had ad libitum access to food and were not manipulated throughout the experiment. W and WRF rats were placed in a rotating drum (1 revolution per 3 min) for 8 h (9 am–5 pm, i.e., ZT2 to ZT10), Monday-to-Friday, for 5 consecutive weeks to simulate shift work, and were maintained unrestrained in their cages over the weekend. W rats had ad libitum access to food at all times, including during work, whereas WRF rats only had access to food outside the work schedule, i.e., from 5 pm to 9 am. After 5 weeks, rats were euthanized during a week-end schedule (no work) every 4 h with n=3 rats per timepoint and group. Hearts were collected, flash-frozen, and processed afterward for RNA extraction and RNA-Seq library preparation