Fig. 1
ABR thresholds from 22 targeted lines displaying non-segregating hearing loss. ABR thresholds showing individual mice from targeted mutant lines displaying hearing loss (filled symbols) which does not segregate with the targeted mutant allele (red, inverted triangles) from the original ABR screen. Mice with normal hearing are shown with empty symbols, and black triangles indicate mice which were wildtype for the targeted mutant allele. Affected mice from the MAKN, MFFD and MUBE lines (top) were almost completely deaf. Affected mice from the MEEK and MEBJ colonies exhibited severe hearing loss, while affected mice from the MBVF colony exhibited varying degrees of hearing loss across all frequencies. Affected mice from the MOAA, MCND, MCFC, MFYJ and MGQK colonies (second and third lines) had hearing loss affecting only the higher frequencies, while the remaining eleven colonies all exhibited hearing loss affecting primarily the low frequencies. The shaded pale green area on each panel denotes the 95% reference range for a large population of control wildtype mice derived from littermates of the tested mice, defined in [11]. All mice were tested at 14 weeks old. Mice from three other lines which exhibited non-segregating hearing loss were detected because of their vestibular phenotype (due to the Tbx1ttch, Pcdh15jigl and Espnspdz alleles) and did not undergo ABRs as part of the ABR screening pipeline. MAKN n = 7 unaffected, 3 affected; MFFD n = 5 unaffected, 1 affected; MUBE n = 4 unaffected, 2 affected; MEEK n = 5 unaffected, 1 affected; MEBJ n = 4 unaffected, 2 affected; MBVF n = 11 unaffected, 6 affected; MOAA n = 3 unaffected, 6 affected; MCND n = 9 unaffected, 5 affected; MCFC n = 7 unaffected, 3 affected; MFYJ n = 3 unaffected, 4 affected; MGKQ n = 9 unaffected, 1 affected; MCRU n = 11 unaffected, 1 affected; MCSJ n = 7 unaffected, 2 affected; METD n = 7 unaffected, 1 affected; MDEU n = 5 unaffected, 2 affected; MCFF n = 16 unaffected, 1 affected; MCBX n = 9 unaffected, 5 affected; MCKG n = 8 unaffected, 2 affected; MCVL n = 7 unaffected, 2 affected; MCTP n = 7 unaffected, 5 affected; MBYL n = 5 unaffected, 1 affected; MATH n = 8 unaffected, 3 affected. Data underlying these plots are in Additional File 3