Fig. 4
From: Fluid shear stress enhances T cell activation through Piezo1
Calcium influx is essential for enhanced T cell activation by FSS in Jurkat cells. A ZAP70 phosphorylation of Jurkat cells treated with FSS and antibodies in calcium-free, or calcium-containing buffer (N=3). B ZAP70 phosphorylation of Jurkat cells treated with FSS and antibodies with or without the calcium chelator EGTA (N=3). C ZAP70 phosphorylation of Jurkat cells treated with FSS and antibodies with or without the mechanosensitive ion channel blocker GsMTx-4 (N=3). D ZAP70 phosphorylation of Cas9 control Jurkat cells and Piezo1 KO Jurkat cells treated with FSS and antibodies (N=3). E ZAP70 phosphorylation of Jurkat cells treated with FSS and antibodies pretreated with 10 μM CCD for 30 min (N=3). F NF-κB phosphorylation of Jurkat cells treated with FSS and antibodies pretreated with 5 μM CSA for 30 min (N=3). Unpaired t tests were used to measure statistical significance between treatment groups. * p<0.05, ** p<0.01, *** p<0.005, **** p<0.001. Error bars are SD