Fig. 2

ChIP-quantitative polymerase chain reaction validation of ChIP-Seq targets enriched in H4ac. A ChIP-qPCR analysis for five genes identified with enriched H4ac following MeJA treatment plus one housekeeping gene (ACTIN2). Relative H4ac enrichment calculated as log2 fold change of H4ac as percentage of input against percentage input without H4ac antibody probing. ChIP-Seq enrichment against input for the same genes is also shown for comparison. Primers were designed in correspondence of the genomic regions most highly enriched in histone markers. Mean values and standard error of the mean (SEM) shown for three and two independent biological replicates for qPCR and ChIP-Seq analysis (Illumina), respectively. Statistical comparison of MeJA-treated and MeJA-untreated samples carried out on ChIP-qPCR data using Welch’s two-sample t-test. B H4ac-enriched ChIP-Seq peaks associated with genes of interest for one representative replicate. Visualized using Integrated Genome Browser [78]