Fig. 6

Spatial co-location analysis and reconstruction of neural morphology in 3D after AKS clearing. A–E Visualisation of spatial co-location of Fluoro-Gold- and YFP-labelled neurons in the cortex. Representative of n = 3 slices each conditioning. A Injection of Fluoro-Gold into Thy1-YFP mice corpus callosum. B, C 3D visualisation of Fluoro-Gold- and YFP-labelled neurons before and after AKS clearing (scale bars: left, XY view, 80 μm; right, XZ view, 50 μm). D, E 3D visualisation of Fluoro-Gold and YFP co-labelled neurons before and after AKS clearing, the enlarged view of the image is obtained from B and C, and the maximum intensity projection (MIP) of the 3D image is shown (scale bars: 30 μm). F–H 3D morphological reconstruction of astrocytes in the hippocampus. Representative of n = 3 slices. F 3D immunostaining of 300-μm-thick Thy1-YFP-H mouse slices with GFAP antibody; maximum intensity projection (MIP) image is shown (scale bars: 50 μm). G 40-μm-thick data cropped from F (scale bars: 40 μm). H 3D reconstruction of astrocytes inside the hippocampus (scale bars: above, 40 μm; below, 10 μm)