Fig. 2

IRISIN promotes beige cell differentiation of mesenchymal stem cells by binding TRPC3. A Protein was isolated from the rosiglitazone- and IRISIN-treated cells and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Protein bands were detected by silver staining. Preliminary screening revealed TRPC3 as an interacting protein. B Screening of 293T cells after transfection with IRISIN and TRPC3 plasmids by co-immunoprecipitation. C TRPC3 protein expression was detected by western blotting. D Western blotting analysis of TRPC3 expression over time. E, F The interaction between TRPC3 and IRISIN was detected using biofilm interference (BLI). The data were analyzed using Data Analysis software 9.0