Fig. 7

ERK/AKT signaling pathway promotes the entry of phosphorylated PPARγ into the nucleus. A Upon IRISIN stimulation, treatment with the inhibitors of PKA, ERK, AKT, and co-treatment with the inhibitors of ERK and AKT, phosphorylation of PPARγ was observed by confocal microscopy. B Phosphorylation of the proteins was detected by western blotting. Expression of phosphorylated PPARγ protein with PYR3 inhibitor. C Phosphorylation of the proteins was detected by western blotting. Expression of phosphorylated PPARγ protein with the inhibitors of PKA, ERK, AKT, and co-treatment with the inhibitors of ERK and AKT. D Western blotting was used to detect the expression of beige fat marker genes UCP1, PGC-1α, and PRDM16