Fig. 2

Tethered drive constructs. a The TARE drive includes a recoded h sequence to provide rescue for h, Cas9 with the nanos promoter/5′UTR and 3′UTR/terminator, EGFP driven by the 3xP3 promoter with a SV40 UTR, and two gRNAs expressed by the U6:3 promoter with tRNAs to separate mature gRNAs. b The TARE drive is inserted into the first exon of the wild-type h allele, located on chromosome 3L, and the drive targets the third coding exon with its gRNAs. c The haplolethal homing drive similarly includes a recoded RpL35A sequence, DsRed, and two gRNAs. d The haplolethal homing drive targets the second exon of the wild-type RpL35A allele, located on chromosome 3R. e The homing suppression drive similarly includes DsRed and four gRNAs. f The homing suppression drive targets the second exon of the wild-type yellow-g allele, located on chromosome 3L