Optimization of the antimicrobial peptide Bac7 by deep mutational scanning

Table 1 Significant pattern mining results

Rank	Position 5	Position 18	Position 19	Position 20	# in 25% *most* actives	# in 25% *least* actives	# in 10% *most* actives	# in 10% *least* actives
1	-	Y	F	M	20	0	20	0
2	-	N	H	N	20	0	20	0
3	C	N	N	-	20	0	19	0
1602	Y	Y	-	Y	11	0	7	0
4815	Y	Y	Y	-	10	0	3	0
32,000	A	P	P	-	0	20	0	20

A subset of triple amino acid combinations was obtained by significant pattern mining (see Additional file 7 for all combinations). The first column indicates the rank of each combination (from most to least significantly enriched in 10% most inhibitory peptides by p-values). Columns 2–5 indicate the amino acid residue at each position in the triple combination. “–” indicates the open fourth position of the peptide. Columns 6–9 indicate the number of peptides containing the respective combination among the top 25% and top 10% most and least active peptides

ISSN: 1741-7007