Fig. 4

Phosphorylated JIP1 rescues the defect in axonal length in JIP1 KO and p35 KO neurons. A Representative images of JIP1 WT and KO neurons expressing GFP, T205A, T205D, and JIP1. Axon length of GFP positive neurons was analyzed 3 days after the infection. Scale bar, 50 μm. B Quantification of axon length relative to GFP control analyzed by two-way ANOVA. Data presented as mean ± SEM; n=233 for GFP, n=230 for T205A, n=231 for T205D, and n=198 for JIP1 for JIP1 WT neurons, and n=224 for GFP, n=227 for T205A, n=97 for T205D, and n=151 for JIP1 for JIP1 KO neurons. “n” represents the number of neurons from one experiment. **** indicates p < 0.0001. C Representative images of axonal length of p35 KO or WT neurons expressing GFP, T205A, T205D, and JIP1 3 days after infection. Scale bar, 50 μm. D Quantification of axonal length relative to GFP control analyzed by two-way ANOVA. Data presented as mean ± SEM; n=87 for GFP, n=85 for T205A, n=81 for T205D, and n=72 for JIP1 for p35 WT neurons, and n=138 for GFP, n=114 for T205A, n=72 for T205D, and n=116 for JIP1 for p35 KO neurons. “n” equals the number of neurons from one experiment and representative of 3 independent experiment. *** p < 0.001 and **** p < 0.0001. E Quantification of axon length in E14.5 embryonic brains subjected to in utero electroporation with GFP and T205D plasmids. Brains were collected and stained with anti-GFP for the measurement of axonal length 3 days after electroporation. Data presented as mean ± SEM. Student’s t-test was used to compare groups of data; n=3 for GFP and T205D for JIP1 WT brains, and n=3 for GFP and n=4 for T205D for JIP1 KO brains. “n” equals the numbers of animals and > 50 GFP-positive cells from each animal were used for quantification. * p < 0.05