Fig. 2

Myeloid differentiation is associated with genome-wide contraction of H3K27me3-marked domains. A Number of H3K27me3-marked genomic regions with widths >100 Kb across cell types indicated by the color legend on the bottom right. B Genome browser view of H3K27me3 density profiles over a 2-Mb window of chromosome 1 highlighting contraction of H3K27me3 in the monocyte (brown) and erythroblast (purple) cells. Maximum value normalized H3K27me3 (C) and H3K4me3 (D) density at coding gene promoters (± 2 Kb of TSS). E Heatmap of H3K27me3 densities colored by cell type at gene promoters and flanks (TSS ± 4 Kb) for regions marked by H3K27me3 in monocytes but not erythroblasts (panel 1), in erythroblast but not monocytes (panel 2), or in both (panel 3). Shading indicates increased density. F Sankey diagram of number of genes retaining or losing H3K27me3 at their gene body in monocytes, erythroblasts, and B and T cells compared to the CD34+CD38− population (dark shade: loss of H3K27me3, light shade retention of H3K27me3). G Percentage of overlap of genes that lost H3K27me3 within upregulated gene bodies in monocytes, erythroblasts, and B and T cells compared to CD34+CD38− cells (the absolute number of overlapping genes is shown on top of each bar). Genome browser view of the H3K27me3 density at CD14 (H), EPB42 (I), and CD36 (J) locus. Expression (RPKM) of each gene across all profiled cell types are shown in the right panel