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Fig. 2 | BMC Biology

Fig. 2

From: Ileum tissue single-cell mRNA sequencing elucidates the cellular architecture of pathophysiological changes associated with weaning in piglets

Fig. 2

Heterogeneity of epithelial secretory cell subtypes in ileum tissues of piglets. a t-SNE plot displaying 832 epithelial secretory cells (ESCs) separated into 6 subtypes. EC, enterocyte; GC, goblet cell; EEC, enteroendocrine cell; B/OC, BEST4/OTOP2 cell. b Violin plots showing canonical marker genes across ESC subtypes. See Additional file 3: Table S2 for all marker genes. c Heatmap reflecting differentially expressed transcription factors and cell-type-specific genes in ESC subtypes. d Immunofluorescence (IF) staining assay of GC marker MUC2 (green) and EEC markers CCK (red) and GCG (red) (n=6); arrow, target cell type; scale bars, 50 μm. e Differentiation pseudotime trajectory analysis of ESC subtypes. Predicted secretory lineage cells include GC, EEC, and B/OC; enterocytes (EC) represent absorptive cells. Red arrow indicates the direction of differentiation. f The differentially expressed genes (rows) along the pseudotime (columns) of secretory and absorptive cells clustering hierarchically into four profiles. The representative gene functions and pathways of each profile are shown

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BMC Biology

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