Fig. 3

In situ localization of the transcription of T. urticae DOG genes. A A schematic representation of a mid-sagittal section of an adult T. urticae female redrafted from Alberti and Crooker [66] with the permission from Elsevier. The digestive and reproductive system is highlighted in dashed red and green lines, respectively. B A virtual mid-sagittal section obtained by a sub-micron CT scan of an adult T. urticae female. The internal morphology is as described by Jonckheere et al. [46]. C A DIG-labeled antisense probe for TuDOG16 was used for hybridization, and the signal was developed using anti-DIG-AP and FastRed as substrate. The reaction product is visible as a red signal (indicating regions where DOGs are expressed) while the spider mite body shows green auto fluorescence. The cuticle stained red in both antisense and sense treatment, so we considered this as a false signal. D The control signal developed from a DIG-labeled sense probe for TuDOG16. C, cuticle; PME, posterior midgut epithelium; OV, ovary; PM, posterior midgut; V, ventriculus; VE, ventricular epithelium; DE, dorsal epithelium; scale bars: 100 μm