Fig. 2

GH5 endoglucanase secreted from Gram-positive bacteria show similar self-truncation process. GH5 endoglucanases Geobacillus sp. 70PC53 GsCelA, Streptococcus sp. SgEGV and Bacillus subtilis strain 168 BsCel5A and Micromonospora (or Thermobispora) GH12 endoglucanase RSC EG1 were incubated in 50 mM sodium acetate, pH 5 at 4 °C for 120 h to assay their self-truncation ability. Four micrograms of purified enzymes were applied to each lane of the gel