Fig. 6

METTL14 mediates m6A modification and controls the expression of multiple phototransduction-related genes. A Heatmap of RNA-seq analysis showing that the levels of several phototransduction-related genes were downregulated in RKO retinas. B RT-qPCR verified the RNA-seq results for the indicated genes (n = 6). C Integrative Genomics Viewer (IGV) tracks displaying MeRIP-seq (lower panels, IP) and RNA-seq (upper panels, input) reads along the locus of phototransduction-related genes in Ctrl and RKO retinas. Two replicates are shown. D Enrichment of m6A at multiple sites (highlighted in Fig. 6C) in transcripts of significant phototransduction-related genes was determined by MeRIP-qPCR. (E–F) Representative immunoblots (E) and quantification (F) of significant phototransduction-related protein levels in retinas from 3-month-old mice. Protein expression data were normalized according to GAPDH (n = 6). *p < 0.05; **p < 0.01; ***p < 0.001. #, no significant difference. Data are presented as the mean ± SEM