Fig. 2

Unlabeled metabolic profiling and analysis of intracellular metabolites of CA-IX expressing MCF-7 cells. A Principal component analysis of intracellular metabolites in CA-IX expressing, MOCK-2, and parental MCF-7 cells. B Heatmap (hierarchical clustering) of the fifty most significant fold changes of intracellular metabolites between CA-IX expressing clones, MOCK-2, and parental cells. Numerous glycolytic intermediates were significantly higher in CA-IX expressing cells compared to MOCK-2 and parental. C–J Average peak intensity of each glycolytic intermediate in CA-IX expressing, MOCK-2, and parental MCF-7 cells. N = 5–6 biological replicates per group, statistical analysis using ordinary one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001