Fig. 6

Expression profiles of lens crystallin genes differ substantially between tadpoles and juveniles. Each of the crystallin genes depicted was significantly differentially expressed between juveniles and tadpoles (adjusted P-value < 0.05; Additional file 1: Table S3). The top row contains crystallins upregulated in tadpoles, which includes two β-crystallins (CRYBA1, CRYBB1), two γ-crystallins (CRYG2, CRYGN), and two taxon-specific crystallins (ρ-crystallin and ζ-crystallin, CRYZ). The bottom row contains those upregulated in juveniles, which includes one α-crystallin (CRYAB), one β-crystallin (CRYBB2), and four γ-crystallins (CRYGB, CRYGB3, CRYG3, CRYG6). Plots show normalized read counts (with an additional pseudocount of 0.5 to allow for log scale plotting) for each gene with light and dark exposed samples denoted by the open and closed circles, respectively. Normalized count data for each gene are found in Additional file 6