Fig. 5

DNA methylation associated with DNMT3A mutations in acute myeloid leukemia. a Scatter plot of DNAm levels in acute myeloid leukemia (AML) with DNMT3A R882 mutation (n = 18) plotted against data of patients without DNMT3A mutations (n = 101). The numbers of CpGs beyond the 20% threshold of differential DNAm are depicted. b Box-whiskers plot (upper and lower quartiles, center line: median, whiskers: 10–90 percentile) showing mean hypo- and hypermethylation of the selected CpGs in (a) in the comparison DNMT3A exon 19^−/− versus wildtype (WT) iHPCs (mean of two biological replica). c Box-whiskers plot of mean hypo- and hypermethylation of the selected CpGs in (a) in the comparison of DNMT3A exon 23^−/− versus WT. d Scatter plot of DNAm levels in AML with DNMT3A mutations other than R882 (n = 15) plotted against data of patients without DNMT3A mutations. e, f Box-whiskers plots of differentially methylated CpGs in AML with non-R882 mutations in DNMT3A in analogy to (b, c). All statistics were performed with 1-way ANOVA and Tukey’s post hoc test