Fig. 6

Competitive growth advantage of exon 23^−/− cells during hematopoietic differentiation. a Syngeneic wildtype, exon 19^−/− and exon 23^−/− iPSCs were transduced with a lentiviral barcoding system and the fluorophores Venus, Cerulean, and mCherry, respectively. The schematic representation depicts competitive growth in co-culture during embryoid body (EB) formation, hematopoietic differentiation, and additional long-term culture (n = 3, all donor 1). b Representative phase contrast and fluorescence microscopic images of EBs with mixed populations at day −4 (day 3 of EB growth). c Flow cytometry analysis of iHPCs after 15 days of hematopoietic differentiation (only cells with a clear fluorescent signal were considered). n = 3 (mean ± SD). d Alternatively, we analyzed the fluorophore-specific genetic barcodes by amplicon sequencing after mixing of the iPSCs (d-7), in EBs (d0), in iHPCs (d15), and after additional long-term expansion (d43). n = 3 (mean ± SD). e Area plots show clonal growth during differentiation and expansion of the three replicates. The graded colors depict corresponding unique molecular identifiers of the lentiviral barcoding. All statistics were performed with 1-way ANOVA and Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001