Fig. 2

Adherens junction defects appear at E15.5 and escalate at E16.5 in Anln-depleted epidermis. A Stereomicroscopic images of E14.5, E15.5, E16.5, and E18.5 embryos infected on E9 with shScr;H2B-GFP (Ctrl) or shAnln-926;H2B-GFP lentiviruses. Arrows denote defects in the head and tail. Scale bar = 2 mm. B Sagittal views of 10-μm sections of dorsal skin from control and shAnln-926 KD E14.5, E15.5, E16.5, and E18.5 embryos. Sections were immunostained for the adhesion receptor E-cadherin. C Dorsal skin sections from embryos treated as in B and immunostained for the polarity protein Par3. D Dorsal skin sections from embryos treated as in B and immunostained for the basement membrane protein nidogen. Dotted lines indicate the dermal–epidermal border. Insets show the transduced cells (H2B−GFP+). Yellow-framed insets show magnification of the boxed area. Nuclei were stained with DAPI (blue). Scale bars = 20 μm