Fig. 7
Functional analyses of the LAL muscle NMJ after nerve crush injury. For functional studies, LAL muscles were analyzed through electrophysiological intracellular recording at the indicated times after nerve crush injury. LAL muscles from age-matched mice were used as controls. a–c After blocking muscle contraction, representative mEPP traces after 0.5 Hz stimuli of NMJs from control and reinnervated muscle fibers and quantification of the amplitude (b) and frequency (c) of mEPPs from muscles from control mice and mice 15, 21, 60, and 90 days after nerve crush. d Representative EPP traces after 0.5 Hz stimuli of NMJs from control and reinnervated muscle fibers. e, f Quantification of EPP amplitude (e) and quantal content (f) at the NMJs of control and nerve-injured mice at the indicated times after nerve crush injury. g Stimulation trains of 5, 10, 20, and 50 Hz showed no changes in paired-pulse facilitation (PPF). h The depression index was calculated after 80 stimuli at 50 Hz. N: number of mice (N: 2 male plus 1 female mice); n: number of fibers (27-37 fibers per mouse). The results were represented as the mean ± SEM, and each individual value for each condition. **p < 0.01; ***p < 0.001; ****p < 0.0001, Mann–Whitney test (b, c, e, f, h), or two-way ANOVA (g)